Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection Geurts and Colleagues, Stem Cell Reports, 2017

Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection

Geurts and Colleagues, Stem Cell Reports 2017

 The potential of CRISPR/Cas9-based genome editing in iPSCs is currently hampered by the laborious nature of identifying cells modified by low frequency homology-directed repair (HDR) pathway.  The Geurts lab presents a straightforward approach, termed co-targeting with selection (CTS), based on co-modification that enriches for cells undergoing HDR thereby improving targeting efficiencies. The CTS method increases the efficiency of genome editing in human iPSCs, enhances detectability of variants of interest derived by HDR, and is a simple, scalable and widely applicable strategy for knocking-in variants of interest.

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